Anti-FZD3 antibody (ab217032)
Key features and details
- Rabbit polyclonal to FZD3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FZD3 antibody
See all FZD3 primary antibodies -
Description
Rabbit polyclonal to FZD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FZD3 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NPG1 -
Positive control
- Mouse pancreas lysate; Rat brain, rat kidney and human colon cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FZD3 antibody (ab217032)Anti-FZD3 antibody (ab217032) at 1/300 dilution + Mouse pancreas lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 76 kDa12% gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling FZD3 with ab217032 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat kidney tissue labeling FZD3 with ab217032 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C. DAPI (5 µg/ml) was used to stain the cell nuclei.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded human colon cancer tissue labeling FZD3 with ab217032 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C. DAPI (5 µg/ml) was used to stain the cell nuclei.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min.
