Anti-IRS1 (phospho S307) antibody (ab1194)
Key features and details
- Rabbit polyclonal to IRS1 (phospho S307)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRS1 (phospho S307) antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 (phospho S307) -
Host species
Rabbit -
Specificity
Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
The 180kDa band is believed to correspond to phospho S307 IRS1. Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.
