Anti-IRS1 antibody (ab52167)
Key features and details
- Rabbit polyclonal to IRS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRS1 antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 -
Host species
Rabbit -
Specificity
ab52167 detects endogenous levels of total IRS1 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IRS1. Synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T).
Database link: P35568
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRS1 antibody (ab52167)All lanes : Anti-IRS1 antibody (ab52167) at 1/500 dilution
Lane 1 : A431 cell extract
Lane 2 : A431 cell extract with immunising peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 132 kDa
Observed band size: >175 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab52167)ab52167 at 1/50 dilution staining IRS1 in human breast carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab52167)ICC/IF image of ab52167 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
