Anti-IRS1 (phospho S307) antibody (ab5599)
Key features and details
- Rabbit polyclonal to IRS1 (phospho S307)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IRS1 (phospho S307) antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 (phospho S307) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human IRS1 aa 301-315.
Sequence: ESITATSpPASMVGGK
(Peptide available asab41770) -
Positive control
- 3T3 L1 cells treated with insulin for 10 minutes. WB: Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysate; NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)All lanes : Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/ml
Lane 1 : Jurkat whole cell lysates
Lane 2 : Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate
Lane 3 : NIH/3T3 whole cell lysates
Lane 4 : NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/ml
Developed using the ECL technique.
Predicted band size: 131 kDa
Observed band size: 131 kDaBlocking buffer: 5% skimmed milk
