Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14357-7] to Cathepsin Z
Suitable for: WB, Flow Cyt, IP
Reacts with: Human

Product name

Anti-Cathepsin Z antibody [EPR14357-7]
See all Cathepsin Z primary antibodies
Description

Rabbit monoclonal [EPR14357-7] to Cathepsin Z
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver and WI-38 lysate. HeLa cells and cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14357-7
Isotype

IgG
Research areas

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/5000 dilution + WI-38 lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 20 µg

Predicted band size: 34 kDa
Observed band size: 34 kDa
Immunoprecipitation - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Western blot analysis of Cathepsin Z in immunoprecipitation pellets from WI-38 lysate immunoprecipitated using ab182575 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow Cytometry - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Cathepsin Z with ab182575 at 1/80 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

All lanes : Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/2000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HeLa lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa
Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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