Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)
![Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)](https://www.abcam.com/ps/products/244/ab244597/Images/ab244597-1-AntiratEndotheliummacrophagesandredbloodcellsantibodyOX43ab243850bone-marrow-cellsFlowCytometry.jpg "Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)")
Key features and details
- Mouse monoclonal [OX-43] to Endothelium, macrophages and red blood cells - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Overview
-
Product name
Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free
See all Endothelium, macrophages and red blood cells primary antibodies -
Description
Mouse monoclonal [OX-43] to Endothelium, macrophages and red blood cells - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Endothelium, macrophages and red blood cells. Resident peritoneal cells from F1 rats.
-
Positive control
- Flow Cyt: Lewis rat bone marrow cells. IHC-Fr: Rat Lung
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-43 -
Isotype
IgG1 -
Light chain type
kappa
Images
-
Flow Cytometry - Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)
This data was developed using the same antibody clone in a different buffer formulation (ab243850)
Lewis rat bone marrow cells stained with ab243850 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat bone marrow cells were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab243850) or mouse IgG1κ (ab170190) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with Ter-119 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable cells
-
![Immunohistochemistry (Frozen sections) - Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)](https://www.abcam.com/ps/products/244/ab244597/Images/ab244597-365870-antiendotheliummacrophageandredbloodcellox43ihcfrrtlung5ug.jpg)
Immunohistochemistry (Frozen sections) - Anti-Endothelium, macrophages and red blood cells antibody [OX-43] - BSA and Azide free (ab244597)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab243850).
IHC image of Endothelium, macrophages and red blood cells staining in a section of frozen normal Rat Lung.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab243850 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
