Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23871-271] to Retinoic Acid Receptor alpha - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free
    See all Retinoic Acid Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR23871-271] to Retinoic Acid Receptor alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis tissue lysate; NIH/3T3, PC-12, MCF7, U937 and HEK-203 whole cell lysates. IHC-P: Human testis tissue; Mouse testis tissue; Rat testis tissue. IP: HEK-293 and NIH/3T3 whole cell lysates.

  • General notes

    ab275748 is the carrier-free version of ab275745. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275748 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    All lanes : Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275745, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: human brain, human skeletal muscle (Database:GTExPortal, HPA).

    Exposure time: 103 seconds.

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    All lanes : Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    This data was developed using ab275745, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 70 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

    This data was developed using ab275745, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (2.71 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of human testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

    This data was developed using ab275745, the same antibody clone in a different buffer formulation.

    Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug

    Lane 2: ab275745 IP in HEK-293 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in HEK-293 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

    This data was developed using ab275745, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of rat testis. The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    All lanes : Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (ab275745) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : U937 (human histiocytic lymphoma monocyte) whole cell lysate
    Lane 3 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa



    This data was developed using ab275745, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: Low expression：MOLT-4.(Database:HPA)

    Lane 1: 8 seconds Lane2-4: 70 seconds

    Exposure time: 

  • Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Immunoprecipitation - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

    This data was developed using ab275745, the same antibody clone in a different buffer formulation.

    Retinoic Acid Receptor alpha was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab275745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275745 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab275745 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275745 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

    This data was developed using ab275745, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Retinoic Acid Receptor alpha with ab275745 at 1/1000 (0.542 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on sertoli cells of mouse testis (PMID:16210368). The section was incubated with ab275745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)
    Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] - BSA and Azide free (ab275748)

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