Anti-FHL1 antibody [EPR7520] (ab133661)
![Anti-FHL1 antibody [EPR7520] (ab133661)](https://www.abcam.com/ps/products/133/ab133661/Images/ab133661-147621-anti-fhl1-antibody-epr7520-western-blot.jpg "Anti-FHL1 antibody [EPR7520] (ab133661)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7520] to FHL1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FHL1 antibody [EPR7520]
See all FHL1 primary antibodies -
Description
Rabbit monoclonal [EPR7520] to FHL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in the Human FHL1 protein (UniProt Q13642).
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Positive control
- Human fetal muscle lysate; Human colon and muscle tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.01 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7520 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FHL1 antibody [EPR7520] (ab133661)Anti-FHL1 antibody [EPR7520] (ab133661) at 1/1000 dilution + Human fetal muscle lysate at 10 µg
Secondary
HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR7520] (ab133661)](https://www.abcam.com/ps/products/133/ab133661/Images/ab133661-147620-anti-fhl1-antibody-epr7520-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR7520] (ab133661)Immunohistochemistry analysis of FHL1 in paraffin embedded Human colon tissue labelled with ab133661 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR7520] (ab133661)](https://www.abcam.com/ps/products/133/ab133661/Images/ab133661-147619-anti-fhl1-antibody-epr7520-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR7520] (ab133661)Immunohistochemistry analysis of FHL1 in paraffin embedded Human muscle tissue labelled with ab133661 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FHL1 antibody [EPR7520] (ab133661)](https://www.abcam.com/ps/products/133/ab133661/Images/ab133661-147618-anti-fhl1-antibody-epr7520.jpg)
OI-RD Scanning - Anti-FHL1 antibody [EPR7520] (ab133661) -
![Anti-FHL1 antibody [EPR7520] (ab133661)](https://www.abcam.com/ps/products/133/ab133661/Images/ab133661-5-benefits-of-recombinant-antibodies.png)
Anti-FHL1 antibody [EPR7520] (ab133661)
