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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Plzf antibody [5B3] (ab104854)

Anti-Plzf antibody [5B3] (ab104854)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5B3] to Plzf
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Plzf antibody [5B3]
    See all Plzf primary antibodies
  • Description

    Mouse monoclonal [5B3] to Plzf
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human Plzf expressed in E. Coli.

  • Positive control

    • HeLa whole cell lysate (ab150035). HeLa cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR161367-22 are from Tissue Culture Supernatant

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5B3
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Images

  • Western blot - Anti-Plzf antibody [5B3] (ab104854)
    Western blot - Anti-Plzf antibody [5B3] (ab104854)
    Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (ab104854)
    Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (ab104854)
    Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow Cytometry - Anti-Plzf antibody [5B3] (ab104854)
    Flow Cytometry - Anti-Plzf antibody [5B3] (ab104854)
    Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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    Applications: WB

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