Anti-Plzf antibody (ab39354)
Key features and details
- Rabbit polyclonal to Plzf
- Suitable for: WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Plzf antibody
See all Plzf primary antibodies -
Description
Rabbit polyclonal to Plzf -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanZebrafish
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Immunogen
Synthetic peptide corresponding to Human Plzf aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab39353) -
Positive control
- This antibody gave a positive signal in HL60 and Ramos whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanZebrafishAll applications MouseRatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).Target
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Function
Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 534401 Cow
- Entrez Gene: 7704 Human
- Entrez Gene: 235320 Mouse
- Entrez Gene: 353227 Rat
- Entrez Gene: 323269 Zebrafish
- Omim: 176797 Human
- SwissProt: Q05516 Human
- SwissProt: Q802Y8 Zebrafish
see all -
Alternative names
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
Images
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Western blot - Anti-Plzf antibody (ab39354)All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes -
Western blot - Anti-Plzf antibody (ab39354)All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Datasheets and documents
References (8)
ab39354 has been referenced in 8 publications.
- Abd-Elrahman KS et al. Autophagy is increased following either pharmacological or genetic silencing of mGluR5 signaling in Alzheimer's disease mouse models. Mol Brain 11:19 (2018). PubMed: 29631635
- Pimenta MT et al. Relaxin affects cell organization and early and late stages of spermatogenesis in a coculture of rat testicular cells. Andrology 3:772-86 (2015). PubMed: 26041439
- Tang Z et al. Protective effect of vitamin E on methyl methanesulfonate-induced teratozoospermia in adult Sprague-Dawley rats. Mol Med Rep 12:4422-4426 (2015). PubMed: 26062593
- Zhang T et al. G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L. Elife 4:e06734 (2015). Human . PubMed: 25821988
- Kirsch S et al. The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein. Int J Mol Med 33:795-808 (2014). WB . PubMed: 24424509
- Jiao B et al. 8-CPT-cAMP/all-trans retinoic acid targets t(11;17) acute promyelocytic leukemia through enhanced cell differentiation and PLZF/RARa degradation. Proc Natl Acad Sci U S A 110:3495-500 (2013). WB ; Mouse . PubMed: 23382200
- Peppi M et al. A corticosteroid-responsive transcription factor, promyelocytic leukemia zinc finger protein, mediates protection of the cochlea from acoustic trauma. J Neurosci 31:735-41 (2011). Mouse . PubMed: 21228182
- Savage AK et al. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity 29:391-403 (2008). WB ; Mouse . PubMed: 18703361
Images
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Western blot - Anti-Plzf antibody (ab39354)All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
-
Western blot - Anti-Plzf antibody (ab39354)All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
