Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5H12] to AIRE - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-AIRE antibody [5H12] - BSA and Azide free
    See all AIRE primary antibodies

  • Description

    Rat monoclonal [5H12] to AIRE - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse thymus tissue.

  • General notes

    ab252249 is the carrier-free version of ab243169. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling AIRE with ab243169 at 1/500 dilution (3.428µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining in the medulla of mouse thymus tissue is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243169).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling AIRE with ab243169 at 1/500 dilution (3.428µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin.

    Negative control: No staining on mouse spleen (PMID: 18322189).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243169).

  • Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)
    Anti-AIRE antibody [5H12] - BSA and Azide free (ab252249)

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