Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5788] to Integrin alpha 2 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free
    See all Integrin alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5788] to Integrin alpha 2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47-D, HEK293, 293T and A431 cell lysates. Human placenta and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and colon tissue. Flow Cyt: A549 and A431 cells.

  • General notes

    ab221214 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

    Flow Cytometry analysis of A549 cells labelling Integrin alpha 2 with purified ab133557 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Integrin alpha 2 with purified ab133557 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133557).

  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

    Overlay histogram showing A431 cells stained with unpurified ab133557 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133557, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Integrin alpha 2 with unpurified ab133557 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133557).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    OI-RD Scanning - Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133557).

  • Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
    Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)

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