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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-CHAMP antibody (ab169249)

Anti-CHAMP antibody (ab169249)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to CHAMP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CHAMP antibody
    See all CHAMP primary antibodies
  • Description

    Mouse polyclonal to CHAMP
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Human CHAMP aa 1-812.
    Sequence:

    MEAFQELRKPSARLECDHCSFRGTDYENVQIHMGTIHPEFCDEMDAGGLG KMIFYQKSAKLFHCHKCFFTSKMYSNVYYHITSKHASPDKWNDKPKNQLN KETDPVKSPPLPEHQKIPCNSAEPKSIPALSMETQKLGSVLSPESPKPTP LTPLEPQKPGSVVSPELQTPLPSPEPSKPASVSSPEPPKSVPVCESQKLA PVPSPEPQKPAPVSPESVKATLSNPKPQKQSHFPETLGPPSASSPESPVL AASPEPWGPSPAASPESRKSARTTSPEPRKPSPSESPEPWKPFPAVSPEP RRPAPAVSPGSWKPGPPGSPRPWKSNPSASSGPWKPAKPAPSVSPGPWKP IPSVSPGPWKPTPSVSSASWKSSSVSPSSWKSPPASPESWKSGPPELRKT APTLSPEHWKAVPPVSPELRKPGPPLSPEIRSPAGSPELRKPSGSPDLWK LSPDQRKTSPASLDFPESQKSSRGGSPDLWKSSFFIEPQKPVFPETRKPG PSGPSESPKAASDIWKPVLSIDTEPRKPALFPEPAKTAPPASPEARKRAL FPEPRKHALFPELPKSALFSESQKAVELGDELQIDAIDDQKCDILVQEEL LASPKKLLEDTLFPSSKKLKKDNQESSDAELSSSEYIKTDLDAMDIKGQE SSSDQEQVDVESIDFSKENKMDMTSPEQSRNVLQFTEEKEAFISEEEIAK YMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCTICGKAFLLE SLLKNHVAAHGQSLLKCPRCNFESNFPRGFKKHLTHCQSRHNEEANKKLM EALEPPLEEQQI


    Database link: NP_115812.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • CHAMP transfected 293T cell line; HeLa cells.
  • General notes

     This product was previously labelled as C13orf8

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger

Images

  • Western blot - Anti-CHAMP antibody (ab169249)
    Western blot - Anti-CHAMP antibody (ab169249)
    All lanes : Anti-CHAMP antibody (ab169249) at 1 µg/ml

    Lane 1 : CHAMP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 89 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (ab169249)
    Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (ab169249)

    Immunofluorescent analysis of HeLa cells labeling CHAMP with ab169249 at 10 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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