Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Ikaros antibody [EPR13790] (ab191394)

Price and availability

284 784 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Ikaros antibody [EPR13790] (ab191394)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13790] to Ikaros
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

You may also be interested in

Product image
Human ZMYM3 knockout HeLa cell line (ab264671)
Product image
Human PHF23 knockout HEK-293T cell lysate (ab259038)
Product image
Human PGR (Progesterone Receptor) knockout HeLa cell lysate (ab257028)
Product image
Anti-GATA4 antibody (ab84593)

Overview

  • Product name

    Anti-Ikaros antibody [EPR13790]
    See all Ikaros primary antibodies
  • Description

    Rabbit monoclonal [EPR13790] to Ikaros
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, Daudi, Raji, Ramos and MOLT4 cell lysates; Human thymus and mouse spleen tissues; MOLT4 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13790
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Images

  • Western blot - Anti-Ikaros antibody [EPR13790] (ab191394)
    Western blot - Anti-Ikaros antibody [EPR13790] (ab191394)
    All lanes : Anti-Ikaros antibody [EPR13790] (ab191394) at 1/10000 dilution

    Lane 1 : Wild-type Jurkat cell lysate
    Lane 2 : IKZF1 knockout Jurkat cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab191394 observed at 50-70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab191394 was shown to react with Ikaros in wild-type Jurkat cells in western blot with loss of signal observed in IKZF1 knockout sample. Wild-type and IKZF1 knockout Jurkat cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab191394 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] (ab191394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] (ab191394)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling Ikaros with ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Ikaros antibody [EPR13790] (ab191394)
    Western blot - Anti-Ikaros antibody [EPR13790] (ab191394)
    All lanes : Anti-Ikaros antibody [EPR13790] (ab191394) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 58 kDa



    Based on the sequence analysis, ab191394 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] (ab191394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] (ab191394)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ikaros with ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Ikaros antibody [EPR13790] (ab191394)
    Flow Cytometry - Anti-Ikaros antibody [EPR13790] (ab191394)

    Flow Cytometric analysis of MOLT4 cells (paraformaldehyde-fixed, 2%) labeling Ikaros with ab191394 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-Ikaros antibody [EPR13790] (ab191394)
    Anti-Ikaros antibody [EPR13790] (ab191394)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Ikaros antibody [EPR13790] (ab191394)

  •  
  • Product image

    PE Anti-Ikaros antibody [4E9] (ab232924)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-Ikaros antibody - N-terminal (ab180713)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Ikaros antibody [SP108] (ab105228)

    Applications: Flow Cyt, IHC-P

  •  
  • Product image

    Anti-Ikaros antibody (ab231474)

    Applications: IHC-P

  •  
  • Product image

    Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645)

    Applications: Flow Cyt, IHC-P, WB

  •  
  • Product image

    Anti-Ikaros antibody [SP108] - BSA and Azide free (ab236227)

    Applications: Flow Cyt, IHC-P

  •  
  • Product image

    Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-Ikaros antibody - N-terminal (ab229275)

    Applications: ChIP, IP, WB

  •  
  • Product image

    Anti-Ikaros antibody (ab168757)

    Applications: WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.