Anti-Ikaros antibody - N-terminal (ab229275)
Key features and details
- Rabbit polyclonal to Ikaros - N-terminal
- Suitable for: WB, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ikaros antibody - N-terminal
See all Ikaros primary antibodies -
Description
Rabbit polyclonal to Ikaros - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment within Human Ikaros (N terminal). The exact sequence is proprietary. short synthetic peptide that lies within amino acids 1-165 of the human DNA-binding protein Ikaros (IKZF1) isoform
Database link: Q13422 -
Positive control
- WB: Jurkat, Raji and NCI-H929 whole cell lysate/extract. IP: Raji whole cell extract. ChIP: Jurkat chromatin extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ikaros antibody - N-terminal (ab229275)All lanes : Anti-Ikaros antibody - N-terminal (ab229275) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate/extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate/extract
Lane 3 : NCI-H929 whole cell lysate/extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 57 kDa7.5% SDS-PAGE
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ChIP - Anti-Ikaros antibody - N-terminal (ab229275)Cross-linked ChIP was performed with Jurkat (Human T cell leukemia cell line from peripheral blood) chromatin extract and 5 μg of either control rabbit IgG or ab229275. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.
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Immunoprecipitation - Anti-Ikaros antibody - N-terminal (ab229275)Ikaros was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab229275. Western blot was performed from the immunoprecipitate using ab229275 at 1/500 dilution.
Lane 1: Control IgG IP in Raji whole cell extract.
Lane 2: ab229275 IP in Raji whole cell extract.
7.5% SDS-PAGE
