Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13791] to Ikaros - C-terminal
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Ikaros antibody [EPR13791] - C-terminal
    See all Ikaros primary antibodies

  • Description

    Rabbit monoclonal [EPR13791] to Ikaros - C-terminal

  • Host species

    Rabbit

  • Specificity

    Based on the sequence analysis, ab190691 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi, Jurkat , Raji and Ramos cell line lysate. Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    All lanes : Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/1000 dilution

    Lane 1 : Wild-type Jurkat cell lysate
    Lane 2 : IKZF1 knockout Jurkat cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab190691 observed at 50-70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab190691 was shown to react with Ikaros in wild-type Jurkat cells in western blot with loss of signal observed in IKZF1 knockout sample. Wild-type and IKZF1 knockout Jurkat cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab190691 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    Flow Cytometry - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ikaros with purified ab190691 at 1/60 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    All lanes : Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/10000 dilution

    Lane 1 : Raji cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : Ramos cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 58 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Immunofluorescent analysis of 4% paraformaldehyde fixed Jurkat cells labeling Ikaros with ab190691 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and conterstained with DAPI.

  • Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)
    Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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