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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)

Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PPAR delta antibody [EPR18517] - BSA and Azide free
    See all PPAR delta primary antibodies
  • Description

    Rabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PPAR delta full length recombinant protein; Jurkat, K562, HeLa, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain, heart and kidney lysates. IP: NIH/3T3 whole cell lysate.
  • General notes

    Ab223137 is the carrier-free version of ab178866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18517
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Fatty acids
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
    Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)

    PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).

    Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab178866 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178866).

  • Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
    Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)

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    Anti-PPAR delta antibody (ab8937)

    Applications: ICC/IF, WB

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    Anti-PPAR delta antibody (ab23673)

    Applications: ICC/IF, WB

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    Anti-PPAR delta antibody [EPR18517] (ab178866)

    Applications: IP, WB

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    Anti-PPAR delta antibody [EPR5185(2)] (ab173299)

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    Anti-PPAR delta antibody (ab137724)

    Applications: IHC-P, WB

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