Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)
![Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)](https://www.abcam.com/ps/products/248/ab248128/Images/ab248128-388755-anti-comt-antibody-epr6490-western-blot-hek293t-lysates.jpg "Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6490] to COMT - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-COMT antibody [EPR6490] - BSA and Azide free
See all COMT primary antibodies -
Description
Rabbit monoclonal [EPR6490] to COMT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248128 is the carrier-free version of ab126618. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6490 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : COMT knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 24-28 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab126618).
Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.
ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)](https://www.abcam.com/ps/products/248/ab248128/Images/ab248128-2-benefits-of-recombinant-antibodies.png)
Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)
