Anti-PLBD2 antibody [EPR21204] (ab225580)
![Anti-PLBD2 antibody [EPR21204] (ab225580)](https://www.abcam.com/ps/products/225/ab225580/Images/ab225580-288577-IGX4128R31minannotated.jpg "Anti-PLBD2 antibody [EPR21204] (ab225580)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21204] to PLBD2
- Suitable for: ELISA, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PLBD2 antibody [EPR21204]
See all PLBD2 primary antibodies -
Description
Rabbit monoclonal [EPR21204] to PLBD2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human PLBD2 aa 400-550.
Database link: Q8NHP8 -
General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21204 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580)All lanes : Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/ml
Lane 1 : Human heart tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lane 3 : Mouse cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![ELISA - Anti-PLBD2 antibody [EPR21204] (ab225580)](https://www.abcam.com/ps/products/225/ab225580/Images/ab225580-288487-PBLD2-small.jpg)
ELISA - Anti-PLBD2 antibody [EPR21204] (ab225580)Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 µg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 µg x mL-1). OD (background signal subtracted) shown on the y-axis.
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![Anti-PLBD2 antibody [EPR21204] (ab225580)](https://www.abcam.com/ps/products/225/ab225580/Images/ab225580-3-benefits-of-recombinant-antibodies.png)
Anti-PLBD2 antibody [EPR21204] (ab225580)
