Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLBD2 antibody [EPR21204] (ab225580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21204] to PLBD2
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PLBD2 antibody [EPR21204]
    See all PLBD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21204] to PLBD2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human PLBD2 aa 400-550.
    Database link: Q8NHP8

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580)
    Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580)
    All lanes : Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human cerebellum tissue lysate
    Lane 3 : Mouse cerebellum tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • ELISA - Anti-PLBD2 antibody [EPR21204] (ab225580)
    ELISA - Anti-PLBD2 antibody [EPR21204] (ab225580)

    Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 µg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 µg x mL-1). OD (background signal subtracted) shown on the y-axis.

  • Anti-PLBD2 antibody [EPR21204] (ab225580)
    Anti-PLBD2 antibody [EPR21204] (ab225580)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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