Anti-PLBD2 antibody (ab138334)
Key features and details
- Rabbit polyclonal to PLBD2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-PLBD2 antibody
See all PLBD2 primary antibodies -
Description
Rabbit polyclonal to PLBD2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Rabbit, Cow, Dog, Human, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse PLBD2 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q3TCN2 -
Positive control
- This antibody gave a positive signal in both Rat Spleen and Lung tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab138334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa). Target
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Function
Putative phospholipase. -
Tissue specificity
Ubiquitously expressed, with highest levels in heart, brain and liver. -
Sequence similarities
Belongs to the phospholipase B-like family. -
Post-translational
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars. -
Cellular localization
Lysosome lumen. - Information by UniProt
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Database links
- Entrez Gene: 196463 Human
- Entrez Gene: 71772 Mouse
- Entrez Gene: 246120 Rat
- SwissProt: Q8NHP8 Human
- SwissProt: Q3TCN2 Mouse
- SwissProt: Q4QQW8 Rat
- Unigene: 115896 Human
- Unigene: 100065 Mouse
see all -
Alternative names
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
see all
Images
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Western blot - Anti-PLBD2 antibody (ab138334)All lanes : Anti-PLBD2 antibody (ab138334) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 40 kDa (possible cleavage fragment)
Exposure time: 6 minutesThe band observed at 40 kDa could potentially be a cleaved form of PLBD2, representing the 346 amino acid chain called "Putative phospholipase B-like 2 40 kDa form".
The band observed at 15 kDa could potentially be a cleaved form of PLBD2, representing the 81 amino acid chain called "Putative phospholipase B-like 2 15 kDa form".
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138334 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References (0)
ab138334 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PLBD2 antibody (ab138334)All lanes : Anti-PLBD2 antibody (ab138334) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 40 kDa (possible cleavage fragment)
Exposure time: 6 minutesThe band observed at 40 kDa could potentially be a cleaved form of PLBD2, representing the 346 amino acid chain called "Putative phospholipase B-like 2 40 kDa form".
The band observed at 15 kDa could potentially be a cleaved form of PLBD2, representing the 81 amino acid chain called "Putative phospholipase B-like 2 15 kDa form".
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138334 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
