Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLBD2 antibody [EPR21742] (ab216866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21742] to PLBD2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PLBD2 antibody [EPR21742]
    See all PLBD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21742] to PLBD2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937, MCF7, MEF and NIH/3T3 whole cell lysates; human fetal spleen, fetal kidney and fetal brain tissue lysates; Mouse brain, kidney and liver tissue lysates; Rat brain, liver and spleen tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21742

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)
    Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)
    All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
    Lane 2 : Human fetal spleen tissue lysate at 20 µg
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 6 : Human fetal kidney tissue lysate at 20 µg
    Lane 7 : Human fetal brain tissue lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 32/28 kDa (possible cleavage fragment)



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 59 seconds

    Lane 3: 15 seconds.

    Lanes 4 & 5: 3 seconds.

    Lanes 6 & 7: 3 minutes.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

  • Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)
    Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)
    All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?
    Additional bands at: 32/28 kDa (possible cleavage fragment)



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 6 seconds.

    Lanes 4-6: 26 seconds.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

  • Anti-PLBD2 antibody [EPR21742] (ab216866)
    Anti-PLBD2 antibody [EPR21742] (ab216866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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