Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Friday March 05, 2021

Anti-PLBD2 antibody [EPR21742] (ab216866)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21742] to PLBD2
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PLBD2 antibody [EPR21742]
See all PLBD2 primary antibodies
Description

Rabbit monoclonal [EPR21742] to PLBD2
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937, MCF7, MEF and NIH/3T3 whole cell lysates; human fetal spleen, fetal kidney and fetal brain tissue lysates; Mouse brain, kidney and liver tissue lysates; Rat brain, liver and spleen tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21742
Isotype

IgG
Research areas

Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)

All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 2 : Human fetal spleen tissue lysate at 20 µg
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 6 : Human fetal kidney tissue lysate at 20 µg
Lane 7 : Human fetal brain tissue lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 65 kDa
Observed band size: 76 kDa
why is the actual band size different from the predicted?
Additional bands at: 32/28 kDa (possible cleavage fragment)

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2: 59 seconds

Lane 3: 15 seconds.

Lanes 4 & 5: 3 seconds.

Lanes 6 & 7: 3 minutes.

Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

This antibody recognizes both full-length and the N-terminal fragment of PLBD2.
Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)

All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 65 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 32/28 kDa (possible cleavage fragment)

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-3: 6 seconds.

Lanes 4-6: 26 seconds.

Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

This antibody recognizes both full-length and the N-terminal fragment of PLBD2.
Anti-PLBD2 antibody [EPR21742] (ab216866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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