Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E341] to MK2
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-MK2 antibody [E341]
See all MK2 primary antibodies
Description

Rabbit monoclonal [E341] to MK2
Host species

Rabbit
Specificity

ab32567 recognises MAPKAP Kinase-2.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.
Positive control
- HeLa whole cell lysate (ab150035).
General notes
This product was previously labelled as MAPKAP Kinase 2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

E341
Isotype

IgG
Research areas

Western blot - Anti-MK2 antibody [E341] (ab32567)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MK2 antibody [E341] (ab32567)

Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

Predicted band size: 46 kDa
Observed band size: 46 kDa
Western blot - Anti-MK2 antibody [E341] (ab32567)

Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution

Lanes 1 & 3 : UV treated HeLa cells
Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor

Secondary
All lanes : IRDye® 680 LICOR goat anti-rabbit.

Performed under reducing conditions.

Predicted band size: 46 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cross reactivity)

Exposure time: 1 minute

ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).
See Abreview
Anti-MK2 antibody [E341] (ab32567)

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