Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MK2 antibody [E341] (ab32567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E341] to MK2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MK2 antibody [E341]
    See all MK2 primary antibodies

  • Description

    Rabbit monoclonal [E341] to MK2

  • Host species

    Rabbit

  • Specificity

    ab32567 recognises MAPKAP Kinase-2.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

     

     

     This product was previously labelled as MAPKAP Kinase 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
    Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution

    Lanes 1 & 3 : UV treated HeLa cells
    Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor

    Secondary
    All lanes : IRDye® 680 LICOR goat anti-rabbit.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible cross reactivity)


    Exposure time: 1 minute


    ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

    See Abreview

  • Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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