Anti-MK2 (phospho T334) antibody (ab131504)
Key features and details
- Rabbit polyclonal to MK2 (phospho T334)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MK2 (phospho T334) antibody
See all MK2 primary antibodies -
Description
Rabbit polyclonal to MK2 (phospho T334) -
Host species
Rabbit -
Specificity
ab131504 detects endogenous levels of MK2 only when phosphorylated at threonine 334. The immunogen sequence used to produce ab131504 is 90% identical to the corresponding region of MAPKAP Kinase 3MK3, therefore ab131504 may cross react with human MK3 but this has not been confirmed.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human MK2 conjugated to keyhole limpet haemocyanin.
Sequence: PQTpPL
Database link: NP_004750.1 -
Positive control
- HeLa cells; HeLa cells treated with UV; Human breast carcinoma tissue.
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General notes
This product was previously labelled as MAPKAP Kinase 2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131504 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MK2 (phospho T334) antibody (ab131504) at 1/500 dilution
Lane 1 : Untreated HeLa cell extract
Lane 2 : HeLa cell extract treated with UV
Predicted band size: 46 kDa
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling MK2 (phospho T334) with ab131504 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MK2 (phospho T334) with ab131504 at 1/50 dilution (left) or ab131504 preincubated with blocking peptide (right).