Anti-CD137 antibody [EPR20238] (ab209256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20238] to CD137
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CD137 antibody [EPR20238]
See all CD137 primary antibodies -
Description
Rabbit monoclonal [EPR20238] to CD137 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186); Human lymphoma and fetal spleen lysates; H9 whole cell lysate; whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20238 -
Isotype
IgG -
Research areas
Images
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Anti-CD137 antibody [EPR20238] (ab209256) at 1/5000 dilution + His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Human lymphoma lysate
Lane 2 : Human fetal spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
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Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution + H9 (Human cutaneous T Lymphocyte cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 36,40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
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All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Whole cell lysate from untreated Jurkat (Human T cell leukemia cell line from peripheral blood)
Lane 2 : Whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 36,40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression level of CD137 can be induced by TPA and Ionomycin [PMID 14648705].
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
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