Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23218-111] to CD137 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD137 antibody [EPR23218-111] - BSA and Azide free
    See all CD137 primary antibodies

  • Description

    Rabbit monoclonal [EPR23218-111] to CD137 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse CD137 recombinant protein(aa24-187); mouse splenocyte lysate (+/- Con A treatment). ICC/IF: Mouse splenocytes treated with Con A; HEK-293T cells transfected with GFP-tagged CD137 expression vector. Flow cyt: Mouse splenocytes treatedwith Con A. IP: Mouse splenocyte whole cell lysate (Con A treated).

  • General notes

    ab270527 is the carrier-free version of ab243648. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
    Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a GFP-tagged CD137 expression vector -  the GPF tag is shown in Green. The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

  • Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
    Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

    Flow cytometric analysis of Mouse splenocytes (treated with 2.5ug/ml concanavalin A  for 72h) cells labelling CD137 with ab243648 at 1/500 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab243648 (Right), then stained with anti-CD25 conjugated to BV421.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

  • Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
    Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

    CD137 was immunoprecipitated from 0.35 mg mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate with ab243648 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243648 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate 10 μg

    Lane 2: ab243648 IP in Mouse splenocyte (treated as above)  whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243648 in Mouse splenocyte (treated as above) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds.

    CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
    Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in mouse splenocytes treated with Concanavalin A (2.5 ug/ml) for 72 h. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

  • Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
    Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

© 2021 Astana Biomed Group LLP. All rights reserved.