Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) (ab273340)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) (ab273340) can be used to detect Phosphoglycerate Mutase (PGAM) activity in biological samples.
The kit utilizes the ability of PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate,which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with a red probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).
This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2-PG Standard Lyophilized 1 vial PGAM Assay Buffer 1 x 25ml PGAM Cofactor Lyophilized 1 vial PGAM Converter 1 Lyophilized 1 vial PGAM Converter 2 Lyophilized 1 vial PGAM Developer Lyophilized 1 vial PGAM Lysis Buffer 1 x 25ml PGAM Positive Control Lyophilized 1 vial PGAM Substrate Lyophilized 1 vial Red Probe 1 x 200µl
Images
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Example data
Example data.
2-PG Standard Curve (colorimetric assay).
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Example dataExample data.
2-PG Standard Curve (fluorimetric assay).
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Example dataExample data.
PGAM activity in rat liver lysate (0.2µg), HepG2 lysate (0.4µg) and Positive Control.