Anti-Nectin-4 antibody [EPR15613] - BSA and Azide free (ab250990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15613] to Nectin-4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Nectin-4 antibody [EPR15613] - BSA and Azide free
See all Nectin-4 primary antibodies -
Description
Rabbit monoclonal [EPR15613] to Nectin-4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250990 is the carrier-free version of ab189514. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250990 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR15613 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 55 kDa
Additional bands at: 66 kDa (possible glycosylated form)This data was developed using ab189514, the same antibody clone in a different buffer formulation.
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Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution + Human placenta lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 44,55 kDa why is the actual band size different from the predicted?
Additional bands at: 66 kDa (possible glycosylated form)This data was developed using ab189514, the same antibody clone in a different buffer formulation.
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