Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR051F] to CD137 - BSA free
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CD137 antibody [BLR051F] - BSA free
See all CD137 primary antibodies
Description

Rabbit monoclonal [BLR051F] to CD137 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD137. Extracellular domain.
Database link: Q07011
Positive control
- IHC-P: Human tonsil tissue. ICC: HCLM2 cells. WB: HDLM whole cell lysate. IP: HDLM2 whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR051F
Isotype

IgG
Research areas
- Cancer
- Tumor immunology
- Cytokines
- TNF

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab252559 at a 1/200 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
Western blot - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

All lanes : Anti-CD137 antibody [BLR051F] - BSA free (ab252559) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : SUP-M2 (Human cell line) whole cell lysate
Lane 5 : HDML2 (Human cell line) whole cell lysate
Lane 6 : My-La-CD4+ (Human cell line) whole cell lysate
Lane 7 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 28 kDa

Exposure time: 3 minutes

Prepared using NETN lysis buffer.
Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

CD37 was immunoprecipitated from HDLM2 (Human cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252559 used for IP at 6 µl per reaction. For WB 1/1000 dilution.

Lane 1: ab252559 IP in HDLM2 whole cell lysate.
Lane 2: Control IgG IP in HDLM2 whole cell lysate.
Chemiluminescence detection: 30 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

FFPE section of HDLM2 (Human cell line) cells labeling CD37 using ab252559 at a 1/200 dilution in ICC.

Secondary: HRP-conjugated goat anti-rabbit IgG.

Substrate: DAB.
Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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