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Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR051F] to CD137 - BSA free
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD137 antibody [BLR051F] - BSA free
    See all CD137 primary antibodies

  • Description

    Rabbit monoclonal [BLR051F] to CD137 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD137. Extracellular domain.
    Database link: Q07011

  • Positive control

    • IHC-P: Human tonsil tissue. ICC: HCLM2 cells. WB: HDLM whole cell lysate. IP: HDLM2 whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab252559 at a 1/200 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

  • Western blot - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    Western blot - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    All lanes : Anti-CD137 antibody [BLR051F] - BSA free (ab252559) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : SUP-M2 (Human cell line) whole cell lysate
    Lane 5 : HDML2 (Human cell line) whole cell lysate
    Lane 6 : My-La-CD4+ (Human cell line) whole cell lysate
    Lane 7 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 28 kDa


    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

    CD37 was immunoprecipitated from HDLM2 (Human cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab252559 used for IP at 6 µl per reaction. For WB 1/1000 dilution.

    Lane 1: ab252559 IP in HDLM2 whole cell lysate.
    Lane 2: Control IgG IP in HDLM2 whole cell lysate.
    Chemiluminescence detection: 30 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

    FFPE section of HDLM2 (Human cell line) cells labeling CD37 using ab252559 at a 1/200 dilution in ICC.

    Secondary: HRP-conjugated goat anti-rabbit IgG.

    Substrate: DAB.

  • Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
    Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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