Anti-MMP2 antibody [EPR17003-25] (ab181286)
![Anti-MMP2 antibody [EPR17003-25] (ab181286)](https://www.abcam.com/ps/products/181/ab181286/Images/ab181286-313129-anti-mmp2-antibody-epr17003-25-immunoprecipitation.jpg "Anti-MMP2 antibody [EPR17003-25] (ab181286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17003-25] to MMP2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MMP2 antibody [EPR17003-25]
See all MMP2 primary antibodies -
Description
Rabbit monoclonal [EPR17003-25] to MMP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HT1080 whole cell lysate. WB: Human, mouse and rat plasma lysates; Rat serum lysate; Mouse and rat lung lysates; HT1080 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17003-25 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (ab181286)
MMP2 was immunoprecipitated from 0.35 mg HT1080 (human fibrosarcoma cell line) whole cell lysate with ab181286 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181286 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HT1080 whole cell lysate 10 µg (Input).
Lane 2: ab181286 IP in HT1080 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181286 in HT1080 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)](https://www.abcam.com/ps/products/181/ab181286/Images/ab181286-313128-anti-mmp2-antibody-epr17003-25-western-blot.jpg)
Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)Lanes 1-5 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/1000 dilution
Lanes 6-7 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/2000 dilution
Lane 1 : Mouse plasma lysate at 20 µg
Lane 2 : Rat serum lysate at 20 µg
Lane 3 : Mouse lung lysate at 20 µg
Lane 4 : Rat plasma lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 10 µg
Lane 7 : Human plasma lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 3 minutes; Lanes 3-5: 10 seconds; Lanes 6-7: 30 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-MMP2 antibody [EPR17003-25] (ab181286)](https://www.abcam.com/ps/products/181/ab181286/Images/ab181286-3-benefits-of-recombinant-antibodies.png)
Anti-MMP2 antibody [EPR17003-25] (ab181286)
