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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Enzymes MMP

Anti-MMP2 antibody (ab235167)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-MMP2 antibody (ab235167)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to MMP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MMP2 antibody
    See all MMP2 primary antibodies
  • Description

    Rabbit polyclonal to MMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 and U87MG cells. IHC-P: Human metastatic breast carcinoma in lymph node tissue. WB: MMP2 HEK-293 over-expressing lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)

    Immunohistochemical analysis of paraffin-embedded human metastic breast carcinoma tissue in lymph node tissue labeling MMP2 with ab235167 at 1/100 dilution (30 minutes at RT), followed by a ready to use Goat anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)

    Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.
    Panel D: Secondary only control.

  • Western blot - Anti-MMP2 antibody (ab235167)
    Western blot - Anti-MMP2 antibody (ab235167)
    All lanes : Anti-MMP2 antibody (ab235167) at 1/1000 dilution

    Lane 1 : MMP2 HEK-293 (human epithelial cell line from embryonic kidney) over-expressing lysate
    Lane 2 : HEK-293 (empty vector) lysate

    Secondary
    All lanes : Pre-adsorbed goat anti-rabbit IgG (H&L) conjugated to HRP at 1/70000 dilution

    Predicted band size: 73 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% BLOTTO.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)

    Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized U87MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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