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Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17003-25] to MMP2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free
    See all MMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17003-25] to MMP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250476 is the carrier-free version of ab181286. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250476 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)
    Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)
    This data was developed using ab181286, the same antibody clone in a different buffer formulation.MMP2 was immunoprecipitated from 0.35 mg HT1080 (human fibrosarcoma cell line) whole cell lysate with ab181286 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181286 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HT1080 whole cell lysate 10 µg (Input).
    Lane 2: ab181286 IP in HT1080 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181286 in HT1080 whole cell lysate (-). Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)
    Western blot - Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)
    Lanes 1-5 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/1000 dilution
    Lanes 6-7 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/2000 dilution

    Lane 1 : Mouse plasma lysate at 20 µg
    Lane 2 : Rat serum lysate at 20 µg
    Lane 3 : Mouse lung lysate at 20 µg
    Lane 4 : Rat plasma lysate at 20 µg
    Lane 5 : Rat lung lysate at 20 µg
    Lane 6 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 10 µg
    Lane 7 : Human plasma lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 73 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181286, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 3 minutes; Lanes 3-5: 10 seconds; Lanes 6-7: 30 seconds.

  • Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)
    Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (ab250476)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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