Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1184] to MMP2
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-MMP2 antibody [EPR1184]
See all MMP2 primary antibodies
Description

Rabbit monoclonal [EPR1184] to MMP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human MMP2 aa 550-650 (C terminal). The exact sequence is proprietary.
Database link: P08253
Positive control
- WB: HepG2, L6, Raw264.7 and NIH/3T3 cell lysates; fetal heart and human skin tissue lysate ICC/IF: PC-3 cells Flow Cyt: HeLa and PC-3 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1184
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution

Lane 1 : L6 (Rat skeletal muscle myoblast) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
Lane 2 : Mouse liver lysates prepared in RIPA lysis method with 5% NFDM/TBST
Lane 3 : Mouse liver lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
Lane 4 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
Lane 5 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 74 kDa
Observed band size: 69,72 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

The 72 KDa band is pro-MMP2 and the 69 KDa band is active-MMP2 reported by PMID 11489818 and 22190701.

This antibody shows low affinity in detecting mouse liver and HepG2 lysates which are positive for MMP2 reported by PMID 24096707 and 24297510.
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] (ab92536)

Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Flow Cytometry - Anti-MMP2 antibody [EPR1184] (ab92536)

Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified ab92536 at 1/180 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/10000 dilution (purified)

Lane 1 : L6 cell lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 74 kDa
Observed band size: 64,72 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
72kDa: propeptide; 64kDa: active form
Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution (unpurified)

Lane 1 : L6 cell lysate
Lane 2 : Fetal heart lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 74 kDa
Flow Cytometry - Anti-MMP2 antibody [EPR1184] (ab92536)

Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

Anti-MMP2 antibody [EPR1184] (ab92536) at 1/5000 dilution (purified) + human skin at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 74 kDa
Observed band size: 64,72 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
72kDa: propeptide; 64kDa: active form
Anti-MMP2 antibody [EPR1184] (ab92536)

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