Antibodies, Proteins, Kits and Reagents for Life Science

301 536 ₸ Product size

1 ml 301 536 ₸

Order now and get it on Wednesday February 24, 2021

Key features and details

Mouse monoclonal [4D3] to MMP2
Suitable for: WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-MMP2 antibody [4D3]
See all MMP2 primary antibodies
Description

Mouse monoclonal [4D3] to MMP2
Host species

Mouse
Specificity

Binds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence).
Sequence:
TSLGLPPDVQRVD

Database link: P08253
Run BLAST with Run BLAST with
Positive control
- Colon carcinoma. Placental tissue

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

4D3
Isotype

IgG1
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Western blot - Anti-MMP2 antibody [4D3] (ab2462)

400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.
Western blot - Anti-MMP2 antibody [4D3] (ab2462) This image is courtesy of an anonymous Abreview

Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg

Secondary
HRP-conjugate Sheep anti-mouse IgG polyclonal

Developed using the ECL technique.

Performed under reducing conditions.

Observed band size: 72 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds
See Abreview

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