Anti-Annexin-6/ANXA6 antibody (ab31026)
Key features and details
- Rabbit polyclonal to Annexin-6/ANXA6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-6/ANXA6 antibody
See all Annexin-6/ANXA6 primary antibodies -
Description
Rabbit polyclonal to Annexin-6/ANXA6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Annexin-6/ANXA6 aa 1-100. The exact sequence is proprietary.
Database link: P08133 -
Positive control
- IHC-P: Human L8 cells. Liver.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as Annexin VI
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026) This image is courtesy of an anonymous AbreviewAnti-Annexin-6/ANXA6 antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-6/ANXA6 antibody (ab31026)Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin-6/ANXA6 in human liver tissue by Immunohistochemistry.
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)All lanes : Anti-Annexin-6/ANXA6 antibody (ab31026) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)Anti-Annexin-6/ANXA6 antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells
Predicted band size: 76 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
