Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal
See all Annexin-2/ANXA2 primary antibodies
Description

Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Rat

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, 293, HeLa, HepG2, SH-SY5Y, C6, RAW264.7, PC12 and NIH 3T3 cell lysates.
General notes
This product was previously labelled as Annexin A2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13053(2)(B)
Isotype

IgG
Research areas

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ANXA2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : SH-SY5Y cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 39 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab185957 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab185957 was shown to react with Annexin-2/ANXA2 in wild-type HeLa cells in western blot. Loss of signal was observed when ANXA2 knockout sample was used. Wild-type and ANXA2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab185957 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/10000 dilution + HeLa cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa
Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

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