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Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal
    See all Annexin-2/ANXA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, 293, HeLa, HepG2, SH-SY5Y, C6, RAW264.7, PC12 and NIH 3T3 cell lysates.

  • General notes

     

     

     This product was previously labelled as Annexin A2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ANXA2 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab185957 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab185957 was shown to react with Annexin-2/ANXA2 in wild-type HeLa cells in western blot. Loss of signal was observed when ANXA2 knockout sample was used. Wild-type and ANXA2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab185957 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

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