Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
![Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206848-anti-annexin-a2-antibody-epr13052b-western-blot.jpg "Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Annexin-2/ANXA2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P07355 -
Positive control
- HeLa, SKBR-3, K562 and HepG2 whole cell lysate (ab7900); Human liver and prostate tissues; HeLa cells and permeabilized K562 cells.
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General notes
This product was previously labelled as Annexin A2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13052(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Lanes 1-3 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/20000 dilution
Lane 4 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDaExposure time
Lanes 1-3: 1 minute
Lane 4: 3 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206847-anti-annexin-a2-antibody-epr13052b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206845-anti-annexin-a2-antibody-epr13052b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206844-anti-annexin-a2-antibody-epr13052b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunofluorescent analysis of HeLa cells labeling Annexin-2/ANXA2 with ab178677 at 1/100 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206843-anti-annexin-a2-antibody-epr13052b-flow-cytometry.jpg)
Flow Cytometry - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-6-benefits-of-recombinant-antibodies.png)
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
