Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)

Key features and details

  • Mouse monoclonal [N159/5] to Protocadherin Gamma (pan) - C-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal

  • Description

    Mouse monoclonal [N159/5] to Protocadherin Gamma (pan) - C-terminal

  • Host species

    Mouse

  • Specificity

    Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Fusion protein corresponding to Mouse Protocadherin Gamma (pan) aa 800 to the C-terminus (C terminal). The immunogens sequence is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789- 912 and C subfamily amino acids ~818-941).
    Database link: NP_291062.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Rat brain lysate.

  • General notes

    The clone number has been updated from S159-5 to N159/5, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Western blot - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186) at 1/1000 dilution + Rat brain membrane lysate at 10 µg

    Secondary
    Goat Anti-Mouse HRP at 1/200 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)
    Immunocytochemistry/ Immunofluorescence - Anti-Protocadherin Gamma (pan) antibody [N159/5] - C-terminal (ab187186)

    Ab187186 staining Protocadherin gamma (pan) in SK-N-BE cells by Immunocytochemistry/Immunofluorescence (ICC/IF).  Cells were fixed with 4% formaldehyde for 15 minutes at room temperature. Samples were incubated wth pirmary antibody at 1/100 dilution for 60 minutes at room temperature. Phalliodin Texas Red F-Actin (1/1000) was used as a counterstain at 1/1000 dilution. DAPI nuclear counterstain was used at 1/5000 dilution.

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