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Signal Transduction Cytoskeleton / ECM Cell Adhesion Annexins

Anti-Annexin-2/ANXA2 antibody (ab137645)

Anti-Annexin-2/ANXA2 antibody (ab137645)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Annexin-2/ANXA2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Annexin-2/ANXA2 aa 1-46 (N terminal).

  • Positive control

    • WB: HeLa, A549 and HCT 116 whole cell lysates. IHC-P: Human pancreas tissue. ICC/IF: HeLa cells.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as Annexin A2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Annexins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Images

  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Annexin-2/ANXA2 knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab137645)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab137645)

    Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling Annexin-2/ANXA2 with ab137645(Green) at 1/500 dilution. Alpha tubulin filaments were labeled with alpha tubulin antibody (Red) at 1/2000.

  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : Untransfected HEK-293T whole cell extract
    Lane 2 : Annexin 1 transfected HEK-293T whole cell extract
    Lane 3 : Annexin 2 transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa

  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    Western blot - Anti-Annexin-2/ANXA2 antibody (ab137645)
    All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate/extract
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab137645)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab137645)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue, labelling Annexin-2/ANXA2 with ab137645 at 1/100 dilution: X20 magnification (left), X40 magnification (right)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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