Anti-Annexin-2/ANXA2 antibody (ab137645)
Key features and details
- Rabbit polyclonal to Annexin-2/ANXA2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit polyclonal to Annexin-2/ANXA2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human Annexin-2/ANXA2 aa 1-46 (N terminal).
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Positive control
- WB: HeLa, A549 and HCT 116 whole cell lysates. IHC-P: Human pancreas tissue. ICC/IF: HeLa cells.
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General notes
Keep as concentrated solution.This product was previously labelled as Annexin A2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Annexin-2/ANXA2 knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 40 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling Annexin-2/ANXA2 with ab137645(Green) at 1/500 dilution. Alpha tubulin filaments were labeled with alpha tubulin antibody (Red) at 1/2000.
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All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution
Lane 1 : Untransfected HEK-293T whole cell extract
Lane 2 : Annexin 1 transfected HEK-293T whole cell extract
Lane 3 : Annexin 2 transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 40 kDa
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All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate/extract
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 40 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab137645)
Immunohistochemical analysis of paraffin embedded Human pancreas tissue, labelling Annexin-2/ANXA2 with ab137645 at 1/100 dilution: X20 magnification (left), X40 magnification (right)