Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19442] to Annexin A10/ANXA10 + Annexin-11/ANXA11
Suitable for: WB, IHC-P
Reacts with: Rat, Human

Product name

Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442]
See all Annexin A10+Annexin A11 primary antibodies
Description

Rabbit monoclonal [EPR19442] to Annexin A10/ANXA10 + Annexin-11/ANXA11
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Annexin A10 and A11 recombinant proteins; HT-1376, HepG2, K562, Jurkat and BxPC-3 whole cell lysates; Human stomach and pancreas lysates. IHC-P: Human stomach, gastric cancer and bladder cancer tissues; Rat stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Rat Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19442
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in human stomach (PMID: 24024557; PMID: 21175800). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/1000 dilution

Lane 1 : HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : Human stomach lysate
Lane 6 : Human pancreas lysate
Lane 7 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 5-7 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 37,54 kDa
Observed band size: 35,54 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1-4,6,7: 3 minutes; Lane 5: 30 seconds.

Blockind/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed (35kDa) is consistent with what has been described in the literature (PMID: 21175800).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human gastric cancer (PMID:24024557; PMID: 21175800). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/10000 dilution

Lane 1 : His-tagged human Annexin A10 recombinant protein (aa134-324)
Lane 2 : His-tagged human Annexin A11 recombinant protein (aa1-505)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 37,54 kDa
Observed band size: 20,54 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

This product cross-reacts with Annexin A11.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human bladder cancer (PMID: 24024557). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in rat stomach. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)

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