Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)
![Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293392-223848ihc1min.jpg "Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19442] to Annexin A10/ANXA10 + Annexin-11/ANXA11
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442]
See all Annexin A10+Annexin A11 primary antibodies -
Description
Rabbit monoclonal [EPR19442] to Annexin A10/ANXA10 + Annexin-11/ANXA11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human Annexin A10 and A11 recombinant proteins; HT-1376, HepG2, K562, Jurkat and BxPC-3 whole cell lysates; Human stomach and pancreas lysates. IHC-P: Human stomach, gastric cancer and bladder cancer tissues; Rat stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19442 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in human stomach (PMID: 24024557; PMID: 21175800). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293393-223848wb2.jpg)
Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/1000 dilution
Lane 1 : HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : Human stomach lysate
Lane 6 : Human pancreas lysate
Lane 7 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 5-7 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 37,54 kDa
Observed band size: 35,54 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-4,6,7: 3 minutes; Lane 5: 30 seconds.
Blockind/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed (35kDa) is consistent with what has been described in the literature (PMID: 21175800).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293391-223848ihc2min.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human gastric cancer (PMID:24024557; PMID: 21175800). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293394-223848wb1.jpg)
Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/10000 dilution
Lane 1 : His-tagged human Annexin A10 recombinant protein (aa134-324)
Lane 2 : His-tagged human Annexin A11 recombinant protein (aa1-505)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 37,54 kDa
Observed band size: 20,54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This product cross-reacts with Annexin A11.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293390-223848ihc3min.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human bladder cancer (PMID: 24024557). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-293389-223848ihc4min.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in rat stomach. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)](https://www.abcam.com/ps/products/223/ab223848/Images/ab223848-8-benefits-of-recombinant-antibodies.png)
Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848)