Mouse Annexin A2 ELISA Kit (ab210576)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Annexin A2 ELISA Kit
See all Annexin-2/ANXA2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% heart ext 5 3.1% Inter-assay Sample n Mean SD CV% heart ext 3 5.8% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
17 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 90% - 101% Cell culture extracts 112 109% - 114% Tissue Extracts 101 89% - 110% Cell culture media 113 106% - 120% Hep Plasma 84 83% - 86% Cit plasma 87 84% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Annexin A2 ELISA Kit (ab210576) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A2 protein in cell culture supernatant, plasma, serum, tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Annexin A2 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Annexin A2 is a secreted 36 kDa calcium-dependent phospholipid-binding protein localized on the surface of most eukaryotic cells. Annexin A2 is involved in several biological processes, including anti-inflammatory effects, Ca2+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. Annexin A2 forms a heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of Annexin A2/p36. Annexin A2 binds two calcium ions with high affinity. Annexin A2 inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Annexin A2 Capture Antibody 1 x 600µl 10X Mouse Annexin A2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Annexin A2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Alternative names
- Annexin A2
- Annexin II
- Annexin II, heavy chain
see all -
Database links
- Entrez Gene: 12306 Mouse
- SwissProt: P07356 Mouse
- Unigene: 238343 Mouse
Images
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Other - Mouse Annexin A2 ELISA Kit (ab210576)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Annexin A2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Annexin A2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Annexin A2 in mouse serum and plasma samples.The concentrations of Annexin A2 were measured in duplicates, interpolated from the Annexin A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Annexin A2 in mouse NIH/3T3 cell extract samples based on 1 µg/mL extract load and in mouse heart extract samples based on 25 µg/mL extract load.The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 5,627 pg/mL in NIH/3T3 cell extract and 8,735 pg/mL in mouse heart extract samples.
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Interpolated concentrations of native Annexin A2 in mouse lung day 5 cell culture supernatant sample.The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 6,362 pg/mL in neat mouse lung supernatant sample.
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Sandwich ELISA - Mouse Annexin A2 ELISA Kit (ab210576)To learn more about the advantages of recombinant antibodies see here.
