Mouse Annexin A2 Matched Antibody Pair Kit (ab218792)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse Annexin A2 Matched Antibody Pair Kit
See all Annexin-2/ANXA2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
9.13 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Annexin A2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Annexin A2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210576. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Annexin A2 Capture Antibody 2 x 50µg 1 x 50µg Mouse Annexin A2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Annexin A2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Alternative names
- Annexin A2
- Annexin II
- Annexin II, heavy chain
see all -
Database links
- Entrez Gene: 12306 Mouse
- SwissProt: P07356 Mouse
- Unigene: 238343 Mouse
Images
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Mouse Annexin A2 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Annexin A2 Matched Antibody Pair Kit (ab218792)To learn more about the advantages of recombinant antibodies see here.
