Human Anti-Brucella IgG ELISA Kit (ab108712)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
-
Product name
Human Anti-Brucella IgG ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos.Serum 23 5.3% Pos.Serum 24 4.2% Inter-assay Sample n Mean SD CV% Pos.Serum 12 3.6% Pos.Serum 12 4.8% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.
A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Brucella (antigen) Coated Microplate (12 x 8 wells) 12 Strips of 8 wells 1 unit Brucella anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Brucella IgG Cut-off Control Yellow, Green cap 1 x 3ml Brucella IgG Negative Control Yellow, Blue cap 1 x 2ml Brucella IgG Positive Control Yellow, Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
-
Alternative names
- B. abortus IgG
- B. canis IgG
- B. melitensis IgG
see all
Images
-
Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
