Anti-Galectin 3 antibody - C-terminal (ab227949)
Key features and details
- Rabbit polyclonal to Galectin 3 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Galectin 3 antibody - C-terminal
See all Galectin 3 primary antibodies -
Description
Rabbit polyclonal to Galectin 3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human Galectin 3 (C terminal). The exact sequence is proprietary.
Database link: P17931 -
Positive control
- WB: HeLa, A549 and HCT 116 whole cell extracts; HEK-293T transfected with Galectin 3 whole cell lysate; Rat colon lysate. IHC-P: Human gastric cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Galectin 3 antibody - C-terminal (ab227949) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Galectin 3 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa
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All lanes : Anti-Galectin 3 antibody - C-terminal (ab227949) at 1/500 dilution
Lane 1 : WT HeLa (epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Galectin 3 knockout HeLa (epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 26 kDa12% SDS-PAGE gel.
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Paraffin-embedded human gastric cancer tissue stained for Galectin 3 using ab227949 at 1/200 dilution in immunohistochemical analysis.
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Anti-Galectin 3 antibody - C-terminal (ab227949) at 1/1000 dilution + Rat colon lysate at 50 µg
Predicted band size: 26 kDa12% SDS-PAGE gel.
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All lanes : Anti-Galectin 3 antibody - C-terminal (ab227949) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate)
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate)
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS PAGE gel.
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All lanes : Anti-Galectin 3 antibody - C-terminal (ab227949) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Galectin 3, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS PAGE gel.