Anti-Galectin 3 antibody [5C21] (ab278071)
![Anti-Galectin 3 antibody [5C21] (ab278071)](https://www.abcam.com/ps/products/278/ab278071/Images/ab278071-445780-AntiGalectin-3-antibody-5C21-ICC-cortical-neuronglial-cell-culture-from-E20-rat.JPG "Anti-Galectin 3 antibody [5C21] (ab278071)")
Key features and details
- Mouse monoclonal [5C21] to Galectin 3
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Galectin 3 antibody [5C21]
See all Galectin 3 primary antibodies -
Description
Mouse monoclonal [5C21] to Galectin 3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Galectin 3.
Database link: P17931 -
Positive control
- WB: Mouse brain lysate; rat brain, liver, kidney, lung. HEK-293 cell lysate. ICC: Embryonic rat (E20) cortical neuron-glial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5C21 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Galectin 3 antibody [5C21] (ab278071)
Cortical neuron-glial cell culture from E20 rat stained for galectin 3 (red) using ab278071 at 1/1000 dilution in immunocytochemistry. A chicken polyclonal antibody to MAP2 (green) was used at 1/10000 dilution. The blue is Hoechst staining of nuclear DNA.
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![Western blot - Anti-Galectin 3 antibody [5C21] (ab278071)](https://www.abcam.com/ps/products/278/ab278071/Images/ab278071-445779-AntiGalectin-3-antibody-5C21-WB-brain-liver-kidney-lung-and-HEK293-cell-lysate.JPG)
Western blot - Anti-Galectin 3 antibody [5C21] (ab278071)Lanes 2-8 : Anti-Galectin 3 antibody [5C21] (ab278071) at 1/2000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain
Lane 4 : Rat liver
Lane 5 : Rat heart
Lane 6 : Rat kidney
Lane 7 : Rat lung
Lane 8 : HEK-293 cell lysate
Predicted band size: 26 kDaThe band at about 30kDa mark corresponds to the galectin 3 protein. The same blot was simultaneously probed with chicken polyclonal to HSP60 (red), at 1/20000 dilution which reveals HSP60 protein in all preparations.