Mouse Galectin-3 Matched Antibody Pair Kit (ab214481)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Mouse Galectin-3 Matched Antibody Pair Kit
See all Galectin 3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.28 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Galectin-3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Galectin-3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab203369. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Galectin-3 Capture Antibody 2 x 50µg 1 x 50µg Mouse Galectin-3 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Galectin-3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
Database links
- Entrez Gene: 16854 Mouse
- SwissProt: P16110 Mouse
- Unigene: 248615 Mouse
Images
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Mouse Galectin-3 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Galectin-3 Matched Antibody Pair Kit (ab214481)To learn more about the advantages of recombinant antibodies see here.
