Anti-CD24 antibody [EPR19925] (ab202073)
![Anti-CD24 antibody [EPR19925] (ab202073)](https://www.abcam.com/ps/products/202/ab202073/Images/ab202073-292942-anti-cd24-antibody-epr19925-immunofluorescence.jpg "Anti-CD24 antibody [EPR19925] (ab202073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19925] to CD24
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD24 antibody [EPR19925]
See all CD24 primary antibodies -
Description
Rabbit monoclonal [EPR19925] to CD24 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19925 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR19925] (ab202073)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) and MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling CD24 with ab202073 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on MCF7 cell line.
Negative control: MDA-MB-231 (PMID: 18433506).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry - Anti-CD24 antibody [EPR19925] (ab202073)](https://www.abcam.com/ps/products/202/ab202073/Images/ab202073-292941-anti-cd24-antibody-epr19925-flow-cytometry.jpg)
Flow Cytometry - Anti-CD24 antibody [EPR19925] (ab202073)Flow cytometric analysis of MDA-MB-231 (human breast adenocarcinoma cell line, Left) and MCF7 breast adenocarcinoma cell line, Right) cell lines labeling CD24 with ab202073 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on total viable cells. Negative control: MDA-MB-231 (PMID: 18433506 and 22934288).
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![Anti-CD24 antibody [EPR19925] (ab202073)](https://www.abcam.com/ps/products/202/ab202073/Images/ab202073-4-benefits-of-recombinant-antibodies.png)
Anti-CD24 antibody [EPR19925] (ab202073)
