Anti-AIRE antibody [5H12] (ab243169)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [5H12] to AIRE
- Suitable for: IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-AIRE antibody [5H12]
See all AIRE primary antibodies -
Description
Rat monoclonal [5H12] to AIRE -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse thymus tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5H12 -
Isotype
IgG2c -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling AIRE with ab243169 at 1/500 dilution (3.428µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining in the medulla of mouse thymus tissue is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling AIRE with ab243169 at 1/500 dilution (3.428µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin.
Negative control: No staining on mouse spleen (PMID: 18322189).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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