Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y1175)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-VEGF Receptor 2 (phospho Y1175) antibody
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 2 (phospho Y1175) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1175). A phospho specific peptide corresponding to residues surrounding Y1175 of Human VEGF Receptor 2.
Database link: P35968 -
Positive control
- WB: HUVEC treated with VEGF cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: MCF-7 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
Lane 1 : HUVEC treated with VEGF cell lysate
Lane 2 : Untreated HUVEC cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG H+L (HRP) at 1/10000 dilution
Predicted band size: 152 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Blocking buffer: 3% BSA
-
Immunofluorescence analysis of methanol-fixed MCF cells, labeling VEGF Receptor 2 (phospho Y1175) (red) with ab194806 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling VEGF Receptor 2 (phospho Y1175) with ab194806 at 1/50 dilution.