Anti-Annexin-2/ANXA2 antibody (ab235939)
Key features and details
- Rabbit polyclonal to Annexin-2/ANXA2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit polyclonal to Annexin-2/ANXA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Guinea pig, Cow, Dog, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human Annexin-2/ANXA2 aa 1 to the C-terminus.
Database link: P07355 -
Positive control
- WB: K562, HeLa, HEK-293, NIH/3T3, MCF7, A549, PC-3 and U-251 MG whole cell lysates; Mouse lung, liver and kidney tissue lysates. IHC-P: Human lung, placenta, liver cancer, and colon cancer tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate (ab150035).
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General notes
This product was previously labelled as Annexin A2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin-2/ANXA2 antibody (ab235939)All lanes : Anti-Annexin-2/ANXA2 antibody (ab235939) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 6 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 7 : Mouse lung tissue lysate
Lane 8 : Mouse liver tissue lysate
Lane 9 : Mouse kidney tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab235939)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin-2/ANXA2 (green) using ab235939 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab235939)Paraffin-embedded human liver cancer tissue stained for Annexin-2/ANXA2 using ab235939 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab235939)Paraffin-embedded human colon cancer tissue stained for Annexin-2/ANXA2 using ab235939 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-Annexin-2/ANXA2 antibody (ab235939)All lanes : Anti-Annexin-2/ANXA2 antibody (ab235939) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab235939)Paraffin-embedded human placenta tissue stained for Annexin-2/ANXA2 using ab235939 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab235939)Paraffin-embedded human lung tissue stained for Annexin-2/ANXA2 using ab235939 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Annexin-2/ANXA2 antibody (ab235939)Annexin-2/ANXA2 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. For western blotting, am HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Lane 1: Rabbit monoclonal IgG (1 µg) instead of ab235939 in Hela whole cell lysate.
Lane 2: ab235939 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (20 μg) (input).
