Anti-TRIB3 antibody [EPR3151Y] (ab75846)
![Anti-TRIB3 antibody [EPR3151Y] (ab75846)](https://www.abcam.com/ps/products/75/ab75846/Images/ab75846-414665-anti-trib3-antibody-epr3151y-western-blot-hct116-wt-trib3-knockout-hct116-hap1-hela.jpg "Anti-TRIB3 antibody [EPR3151Y] (ab75846)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3151Y] to TRIB3
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TRIB3 antibody [EPR3151Y]
See all TRIB3 primary antibodies -
Description
Rabbit monoclonal [EPR3151Y] to TRIB3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT116 cell lysates; 293T cell lysates, untreated and treated with Ionomycin and TPA.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3151Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution
Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : TRIB3 knockout HCT116 cell lysate
Lane 3 : Hap1 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDaLanes 1 - 4: Merged signal (red and green). Green - ab75846 observed at 40 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab75846 was shown to react with TRIB3 in HCT 116 wild-type cells in western blot with loss of signal observed in TRIB3 knockout cell line ab273718 (TRIB3 knockout cell lysate ab275249). HCT 116 wild-type and TRIB3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75846 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)](https://www.abcam.com/ps/products/75/ab75846/Images/ab75846.gif)
Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution
Lane 1 : 293T cell lysates, treated with Ionomycin and
TPA
Lane 2 : 293T cell lysates, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
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![Anti-TRIB3 antibody [EPR3151Y] (ab75846)](https://www.abcam.com/ps/products/75/ab75846/Images/ab75846-3-benefits-of-recombinant-antibodies.png)
Anti-TRIB3 antibody [EPR3151Y] (ab75846)
